Heteronuclear Single-Quantum Coherence (HSQC) Spectra

Examples of 1H-15N HSQC spectra
  • (A) decoupled spectrum, signals corresponding to different spin transitions are overlapping
  • (B) coupled spectrum, all four signals in the 1H-15N spin pair are resolved

       Details:

  • Pulse sequence: standard Bruker pulse sequence invitp

    • (A)decoupling is applied during 15N evolution period and during acquisition; (B) no decoupling
  • Spin system: two nuclei: 1H at 8.0 ppm, 15N at 117.0 ppm; scalar coupling: JNH=90 Hz

    • spin relaxation rates were calculated for each spin transition using our program DYNAMICS
    (this spin system corresponds to a typical backbone amide group in proteins)
  • Spectrometer frequency: 600 MHz (1H)
  • Acquisition: 32 x 64 complex points
  • Processing details: zero filling to 64 x 64 points, apodization using 90o shifted sinebell, TPPI method
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